منابع مشابه
Improving peptide identification with Percolator
Bacterial infections require several stages, each mediated by different bacterial virulence genes. Studying the function of these important genes during infection is hampered by the difficulty of modulating bacterial gene expression in an infected animal. Eduardo Santero and colleagues describe the incorporation of a gene expression control circuit into bacteria that respond to salicylate or ac...
متن کاملHigh hydrostatic pressure
The use of high pressure processing (HPP) in the food industry was developed as an alternative to common thermal processes, such as pasteurization and sterilization, to produce a microbiologically safe food while avoiding and reducing undesirable changes in sensory, physiochemical and nutritional properties of foods. The use of high pressure at high temperatures as a tool for sterilization (hig...
متن کاملFast and Accurate Protein False Discovery Rates on Large-Scale Proteomics Data Sets with Percolator 3.0
Percolator is a widely used software tool that increases yield in shotgun proteomics experiments and assigns reliable statistical confidence measures, such as q values and posterior error probabilities, to peptides and peptide-spectrum matches (PSMs) from such experiments. Percolator's processing speed has been sufficient for typical data sets consisting of hundreds of thousands of PSMs. With o...
متن کاملImprovements to the percolator algorithm for Peptide identification from shotgun proteomics data sets.
Shotgun proteomics coupled with database search software allows the identification of a large number of peptides in a single experiment. However, some existing search algorithms, such as SEQUEST, use score functions that are designed primarily to identify the best peptide for a given spectrum. Consequently, when comparing identifications across spectra, the SEQUEST score function Xcorr fails to...
متن کاملChaperone-percolator model: a possible molecular mechanism of Anfinsen-cage-type chaperones.
Although we have a rather elaborate "working-cycle" for the 60 kDa molecular chaperones, which possess a cavity, and are called Anfinsen-cage-type chaperones to emphasize that they provide a closed, protected environment to help the folding of their substrates, our understanding of the molecular mechanism of how these chaperones help protein folding is still incomplete. The present study adds t...
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ژورنال
عنوان ژورنال: Minutes of the Proceedings of the Institution of Civil Engineers
سال: 1854
ISSN: 1753-7843
DOI: 10.1680/imotp.1854.23954